SHENDY,, M., EL-DAKHLY, A., YOUSSEF, M. (2017). PREPARATION OF A THERMOSTABLE BOVINE EPHEMERAL FEVER VIRUS VACCINE INACTIVATED ON THE TIME OF USE. Egyptian Journal of Agricultural Research, 95(2), 933-940. doi: 10.21608/ejar.2017.148933
MOHAMED B. SHENDY,; ASHRAF T. EL-DAKHLY; MOHAMED M. YOUSSEF. "PREPARATION OF A THERMOSTABLE BOVINE EPHEMERAL FEVER VIRUS VACCINE INACTIVATED ON THE TIME OF USE". Egyptian Journal of Agricultural Research, 95, 2, 2017, 933-940. doi: 10.21608/ejar.2017.148933
SHENDY,, M., EL-DAKHLY, A., YOUSSEF, M. (2017). 'PREPARATION OF A THERMOSTABLE BOVINE EPHEMERAL FEVER VIRUS VACCINE INACTIVATED ON THE TIME OF USE', Egyptian Journal of Agricultural Research, 95(2), pp. 933-940. doi: 10.21608/ejar.2017.148933
SHENDY,, M., EL-DAKHLY, A., YOUSSEF, M. PREPARATION OF A THERMOSTABLE BOVINE EPHEMERAL FEVER VIRUS VACCINE INACTIVATED ON THE TIME OF USE. Egyptian Journal of Agricultural Research, 2017; 95(2): 933-940. doi: 10.21608/ejar.2017.148933
PREPARATION OF A THERMOSTABLE BOVINE EPHEMERAL FEVER VIRUS VACCINE INACTIVATED ON THE TIME OF USE
Veterinary Serum and Vaccine Research Institute. Abbasia. Cairo.
Abstract
the herein study was devoted to overcome the obstacles dealing with the utilization of the live attenuated Bovine Ephemeral Fever (BEF) vaccine viz, the cold chain and the undesirable post-vaccinal reaction. Methylglucoside-sucrose (MS) was proved to be an excellent stabilizer for BEF thermostable vaccine. The permissible titer of the lyophilized MS-BEF thermostable vaccine was achieved even after 6 months at 25°C, 3 months at 37°C and 20 days at 45°C. The study revealed that freeze-dried MS-BEF thermostable vaccine was reconstituted with saponine (0.2μg/dose) as inactivator just at the time of inoculation. Such investigations were carried out on 4 groups of calves each was consisted of three animals. The first group of animals were inoculated with the MS-BEF thermostable vaccine kept at 25°C for 6 months. The second groups were inoculated with the forementioned vaccine kept at 37°C for 3 months. The third group of animals were vaccinated with BEF vaccine kept at 45 for 20 days. (The vaccine titer was 105.5 TCID50/ml in the three above mentioned groups), whereas the fourth group was kept as nonvaccinated normal controls. The three animal groups received booster dose at two weeks of post-first vaccination. It has been deduced that such vaccine effectively induced high mean BEF neutralizing antibody titers post- boostering vaccination and protected cattle for one year. Thus, MS stabilizer was recommended for the preparation of thermostable live BEF vaccine inactivated at the spot with saponine.