CHANGES IN ENZYMATIC ACTIVITIES OF THE PINK BOLLWORM LARVAE TREATED WITH SOME BIOPESTICIDES

Document Type : Original Article

Authors

1 Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt.

2 Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, Egypt.

Abstract

The present investigation aimed to clarify the changes in the enzymatic activities of the Pinkbollworm larvae treated with some biopesticides. Data showed that Abamectin( 1.8%EC ) is the most effective than Spinosad (24%SL ) on the third instar larvae of the pink bollworm (pectinophora gossypiella) (Saund) followed by Esfenvalerate (20%EC) and Dipel 2x (6.4%WP) (Bacillus thuringiensis) respectively. The LC50 of Abamectin, Spinosad, Esfenvalerate and Dipel 2x caused increasing effect in protein concentration, reaching 28.8, 25, 48.9 and 39.1% respectively in gut tissue of larvae after 6 hrs. of treatment. After 24 and 48 hrs. of treatment this content was decreased in Dipel 2x and Abamectin by 33.1, 33.5% and 39, 26.3% respectively. But the treatment of Spinosad and Esfenvalerate increased the protein by 26.2, 13.2% and 18, 16.2% respectively. Great increase in activity of protease and glutathione-s-transferase enzymes was recorded in larvae after all intervals of treatment by the tested pesticides. A high inhibitions in the activity of ATPase in larval tissues after all intervals of treatment except Abamectin and Esfenvalerate after 48 hrs. of treatment were occurred. A Significant increase in acid and alkaline phosphatase activities was detected in 6hrs. treatment of the four insecticides, but this response was changed after 24 and 48hrs. of treatments .

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