Tissue culture inactivated ISA-70 oil adjuvanted EHV-1 vaccine was successfully prepared from the local isolate. The virus was completely inactivated by 0.008 M of binary ethyleneimine (BEI) within 24 hours at 37ºC. Mineral oil (ISA-70) achieves long term immunity & good seringeabilty. The vaccine proved to be safe, immunogenic in the inoculated mice & pregnant mares. It was potent in vaccinated horses with protective level of neutralizing & complements fixing and AGPT antibodies for 5 months. The duration of virus recovery in vaccinated mice after challenge was 4 days compared with 9 days in the control group. The stability of the prepared vaccine was studied whereas it could be stored at 4ºC, and withstand room temperature and 37ºC for 2 years.
WARDA, S. A., & HUSSIEN, G. M. (2012). IMMUNE RESPONSE OF HORSES VACCINATED WITH TISSUE CULTURE INACTIVATED EHV-1 OIL (MONTANIDE ISA-70) ADJUVANTED VACCINE. Egyptian Journal of Agricultural Research, 90(3), 1309-1321. doi: 10.21608/ejar.2012.163006
MLA
SAFAA A. WARDA; GAMAL M. HUSSIEN. "IMMUNE RESPONSE OF HORSES VACCINATED WITH TISSUE CULTURE INACTIVATED EHV-1 OIL (MONTANIDE ISA-70) ADJUVANTED VACCINE", Egyptian Journal of Agricultural Research, 90, 3, 2012, 1309-1321. doi: 10.21608/ejar.2012.163006
HARVARD
WARDA, S. A., HUSSIEN, G. M. (2012). 'IMMUNE RESPONSE OF HORSES VACCINATED WITH TISSUE CULTURE INACTIVATED EHV-1 OIL (MONTANIDE ISA-70) ADJUVANTED VACCINE', Egyptian Journal of Agricultural Research, 90(3), pp. 1309-1321. doi: 10.21608/ejar.2012.163006
VANCOUVER
WARDA, S. A., HUSSIEN, G. M. IMMUNE RESPONSE OF HORSES VACCINATED WITH TISSUE CULTURE INACTIVATED EHV-1 OIL (MONTANIDE ISA-70) ADJUVANTED VACCINE. Egyptian Journal of Agricultural Research, 2012; 90(3): 1309-1321. doi: 10.21608/ejar.2012.163006
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