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GAMAL, R., AL-FATEH, S., MOHAMMAD, A., EL- SAWY, M. (2013). EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD). Egyptian Journal of Agricultural Research, 91(2), 413-427. doi: 10.21608/ejar.2013.163072
RAWIA F. GAMAL; SAMER R. AL-FATEH; ABEER M. MOHAMMAD; MOHAMED EL- SAWY. "EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD)". Egyptian Journal of Agricultural Research, 91, 2, 2013, 413-427. doi: 10.21608/ejar.2013.163072
GAMAL, R., AL-FATEH, S., MOHAMMAD, A., EL- SAWY, M. (2013). 'EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD)', Egyptian Journal of Agricultural Research, 91(2), pp. 413-427. doi: 10.21608/ejar.2013.163072
GAMAL, R., AL-FATEH, S., MOHAMMAD, A., EL- SAWY, M. EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD). Egyptian Journal of Agricultural Research, 2013; 91(2): 413-427. doi: 10.21608/ejar.2013.163072

EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD)

Article 3, Volume 91, Issue 2, June 2013, Page 413-427  XML PDF (367.01 K)
Document Type: Original Article
DOI: 10.21608/ejar.2013.163072
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Authors
RAWIA F. GAMAL1; SAMER R. AL-FATEH2; ABEER M. MOHAMMAD2; MOHAMED EL- SAWY1
1Dept. of Microbiology, Fac. of Agriculture, Ain Shams Univ.
2Plant Protection Research Institute, ARC, Dokki, Giza, Egypt
Abstract
The effect of stress conditions on viability and activity of the recombinant E. coli, that contains cryIAC gene, which encodes a crystal toxin specific to Spodoptera littoralis, was tested. Solar radiation showed the worst effect on both viability and activity against the larvae of S. littoralis. The drastic effect also happened when recombinant E. coli was exposed to heat treatment at 50oC for more than 5 min. and to high concentrations of sucrose (> 20 % for more than 2 days). Also, the same effect happened when cells were exposed to NaCl > 5 %, even after one day of exposure. The viability of E. coli cells after preservation by freezing under glycerol, lyophilization and encapsulation indicated that, freezing was the best method for keeping both viability and activity of cells till 9 months post preparation.
Keywords
ACTIVITY OF A RECOMBINANT; SPODOPTERA LITTORALIS (BOISD
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