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ABDEL-WAHED, M., EL-BERMAWY, S., EL-DOUGDOUG, K., ABDEL-AZIZ, M. (2013). EVALUATION OF ONE BIOAGENT AND AN INSECT GROWTH REGULATOR AGAINST THE COTTON LEAFWORM USING PCR. Egyptian Journal of Agricultural Research, 91(2), 525-537. doi: 10.21608/ejar.2013.163479
MOHAMED S. ABDEL-WAHED; SAADIA M. EL-BERMAWY; KHALED A. EL-DOUGDOUG; MARWA M. ABDEL-AZIZ. "EVALUATION OF ONE BIOAGENT AND AN INSECT GROWTH REGULATOR AGAINST THE COTTON LEAFWORM USING PCR". Egyptian Journal of Agricultural Research, 91, 2, 2013, 525-537. doi: 10.21608/ejar.2013.163479
ABDEL-WAHED, M., EL-BERMAWY, S., EL-DOUGDOUG, K., ABDEL-AZIZ, M. (2013). 'EVALUATION OF ONE BIOAGENT AND AN INSECT GROWTH REGULATOR AGAINST THE COTTON LEAFWORM USING PCR', Egyptian Journal of Agricultural Research, 91(2), pp. 525-537. doi: 10.21608/ejar.2013.163479
ABDEL-WAHED, M., EL-BERMAWY, S., EL-DOUGDOUG, K., ABDEL-AZIZ, M. EVALUATION OF ONE BIOAGENT AND AN INSECT GROWTH REGULATOR AGAINST THE COTTON LEAFWORM USING PCR. Egyptian Journal of Agricultural Research, 2013; 91(2): 525-537. doi: 10.21608/ejar.2013.163479

EVALUATION OF ONE BIOAGENT AND AN INSECT GROWTH REGULATOR AGAINST THE COTTON LEAFWORM USING PCR

Article 11, Volume 91, Issue 2, June 2013, Page 525-537  XML PDF (430.84 K)
Document Type: Original Article
DOI: 10.21608/ejar.2013.163479
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Authors
MOHAMED S. ABDEL-WAHED1; SAADIA M. EL-BERMAWY2; KHALED A. EL-DOUGDOUG1; MARWA M. ABDEL-AZIZ3
1Faculty of Agriculture, Ain Shams University.
2Faculty of Education, Ain Shams University.
3Plant Protection Research Institute, ARC.
Abstract
This study was carried out on 6th instar larvae of Spodoptera littoralis treated in 2nd instar larvae with LC50 of Match and Protecto at 0.0057 ppm, and 0.1099 gm/ml., respectively, Five random primers were used in this study to generate a fragmenting pattern as a tool to investigate the molecular differences between treated samples and control. The numbers of unique and common fragments generated by using these primers (OPO1, OPO2, OPO3, OPO4 and OPO5) was recorded. It has been found that primer OPO2 was the most powerful in generating a unique informative fragmenting pattern, it gave 4 specific unique fragments. The primer OPO4 was the poorest one in generating an informative fragmenting pattern.
Keywords
BIOAGENT AND AN INSECT GROWTH; COTTON LEAFWORM
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