ABDELMAKSOUD, H., MANDOUR, A., GAMAL ELDIN, A. (2013). MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT. Egyptian Journal of Agricultural Research, 91(4), 1259-1269. doi: 10.21608/ejar.2013.165097
HESHAM M. ABDELMAKSOUD; AYMAN M. MANDOUR; AHMED S. GAMAL ELDIN. "MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT". Egyptian Journal of Agricultural Research, 91, 4, 2013, 1259-1269. doi: 10.21608/ejar.2013.165097
ABDELMAKSOUD, H., MANDOUR, A., GAMAL ELDIN, A. (2013). 'MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT', Egyptian Journal of Agricultural Research, 91(4), pp. 1259-1269. doi: 10.21608/ejar.2013.165097
ABDELMAKSOUD, H., MANDOUR, A., GAMAL ELDIN, A. MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT. Egyptian Journal of Agricultural Research, 2013; 91(4): 1259-1269. doi: 10.21608/ejar.2013.165097
MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT
Plant Virology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
Abstract
Potato leaf roll virus (PLRV) was isolated from Egyptian grown potatos. The virus was identified on the basis of host range, symptomatology, insect transmission, electron microscopy, RT-PCR, and PCR-ELISA. The complete nucleotide sequence of genomic PLRV-RNA was obtained from cloned cDNA and submitted in GenBank under Accession No. AY138970. This sequence is 5884 nucleotide long and encodes 5 ORF with an unique read-through protein suggested a conflict with the conceptual translation at amino acid 209. Comparison of PLRV sequence with that of other PLRV strains shows overall similarities of 97.02% and high genetic identity of 100% (Polish Strain), 98.5% (French Strain), 98.4% (Wageningen Strain), 98.3% (UK Strain), 98% (Canadian Strain), and 93. 5% (Australian Strain).
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