IN VITRO CONSERVATION OF DATE PALM SHOOT TIP EXPLANTS UNDER MINIMAL GROWTH CONDITION

Document Type : Original Article

Authors

1 . The Central Laboratory for Date Palm Researches and Development, ARC, Giza

2 Department of Pomology, Faculty of Agriculture, Cairo University, Egypt

3 The Central Laboratory for Date Palm Researches and Development, ARC, Giza

Abstract

This current investigation was conducted to study the effect of different sugar type (sucrose, sorbitol or mannitol at 0.3M) with different ABA concentration (0.0, 2.0, 4.0, 6.0 and 8.0 mg/l) on the shoot tip explants of date palm Zaghlool cv. conserved at 5 or 15 C° under complete darkness for six and 12 months by observing the physiological changes and growth degree of the conserved explants during the conservation period and also their recovery when they were returned and recultured under normal growth condition in order to achieve the best minimal growth condition for in vitro conservation of shoot tip explants. All shoot tip explants conserved at 5 C° for six month or at 15 C° for 12 months on conservation medium supplemented with 0.3M sucrose or sorbitol combined with different concentration of ABA able to survive. Yet, there are few information about conservation of date palm shoot tip explants or embryonic callus by in vitro slow growth storage so this approach needs more studies to keep continuously viable sterilized and contaminated free new source from mother plants for labor commercial production.

Keywords

Files

Share

How to cite

Statistics