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HUSSEIN, E., MANSOUR, M., HASSAN, M., EL-KADY, E., KASEM, K. (2011). USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW. Egyptian Journal of Agricultural Research, 89(1), 17-34. doi: 10.21608/ejar.2011.170370
EZAT M. HUSSEIN; MAHMOUD T. MANSOUR; MAGGIE E.M. HASSAN; EMAN A. EL-KADY; KHALED K. KASEM. "USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW". Egyptian Journal of Agricultural Research, 89, 1, 2011, 17-34. doi: 10.21608/ejar.2011.170370
HUSSEIN, E., MANSOUR, M., HASSAN, M., EL-KADY, E., KASEM, K. (2011). 'USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW', Egyptian Journal of Agricultural Research, 89(1), pp. 17-34. doi: 10.21608/ejar.2011.170370
HUSSEIN, E., MANSOUR, M., HASSAN, M., EL-KADY, E., KASEM, K. USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW. Egyptian Journal of Agricultural Research, 2011; 89(1): 17-34. doi: 10.21608/ejar.2011.170370

USE OF SEROLOGY, SDS-PAGE, AND RAPD ANALYSIS TO EVALUATE RESISTANCE OF FLAX TO POWDERY MILDEW

Article 2, Volume 89, Issue 1, March 2011, Page 17-34  XML PDF (703.87 K)
Document Type: Original Article
DOI: 10.21608/ejar.2011.170370
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Authors
EZAT M. HUSSEIN1; MAHMOUD T. MANSOUR1; MAGGIE E.M. HASSAN1; EMAN A. EL-KADY2; KHALED K. KASEM1
1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
2Field Crops Research Institute, Agricultural Research Center, Giza, Egypt.
Abstract
Eight flax cultivar were evaluated for powdery mildew resistance under field conditions , C.I. 2008, Cortland, Linore and the local cultivars Giza 8 , Giza 7 were highly susceptible. Disease severities on these cultivars were 100.00, 99.48 and 95.26 , 93.46 and 87.26%respectively. On the other hand, cultivars Williston brown, Wilden and Dokota were highly resistant,with disease severities on these cultivars were 27.58, 25.11 and 19.67%, respectively. SDS-PAGE, RAPD analysis by using four primers and double diffusion test (DDT) were used to differentiate between resistance and susceptible genotypes. RAPD analysis by using primer no. 6 and DDT were able, at least partially, to differentiate between resistant and susceptible genotypes, while SDS-PAGE was unable to differentiate between cultivars of the two groups.
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