Local isolate of (IBD) virus [EID50 106/ml] was propagated for three passages on (SPF) embryonated chicken eggs (EID50 106.5/ml) followed by three passages on SPF chickens (EID50 108/ml), then, for hundred and ten serial passages on SPF embryonated chicken eggs prepared and titrated (EID50 107/ml) after every 5 passages. The virus was inoculated into 21 days old chickens and observed for 21 days post-infection, then, challenged with virulent IBD virus. Passages (110, 77, 55) were used for preparation of live attenuated vaccines mild, intermediate, hot (IBDV) vaccines on SPF (ECE), respectively. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency was performed by measuring humoral and cellular immune response, as well as, protection percentage against virulent IBDV.The Efficacy of the prepared vaccine was estimated for up to six months.
IBRAHIM, N. M. (2011). IMMUNOLOGICAL STUDIES ON THE LOCAL INFECTIOUS BURSAL DISEASE VIRUS (IBD) ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS SPF (ECE). Egyptian Journal of Agricultural Research, 89(2), 709-729. doi: 10.21608/ejar.2011.176064
MLA
NADIA M. IBRAHIM. "IMMUNOLOGICAL STUDIES ON THE LOCAL INFECTIOUS BURSAL DISEASE VIRUS (IBD) ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS SPF (ECE)", Egyptian Journal of Agricultural Research, 89, 2, 2011, 709-729. doi: 10.21608/ejar.2011.176064
HARVARD
IBRAHIM, N. M. (2011). 'IMMUNOLOGICAL STUDIES ON THE LOCAL INFECTIOUS BURSAL DISEASE VIRUS (IBD) ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS SPF (ECE)', Egyptian Journal of Agricultural Research, 89(2), pp. 709-729. doi: 10.21608/ejar.2011.176064
VANCOUVER
IBRAHIM, N. M. IMMUNOLOGICAL STUDIES ON THE LOCAL INFECTIOUS BURSAL DISEASE VIRUS (IBD) ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS SPF (ECE). Egyptian Journal of Agricultural Research, 2011; 89(2): 709-729. doi: 10.21608/ejar.2011.176064
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