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HASSAN, E., ABDEL WANIS, N., TOLBA, S. (2011). STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE. Egyptian Journal of Agricultural Research, 89(3), 1101-1158. doi: 10.21608/ejar.2011.177827
EMAN A. HASSAN; NABEL A. ABDEL WANIS; SUSAN K. TOLBA. "STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE". Egyptian Journal of Agricultural Research, 89, 3, 2011, 1101-1158. doi: 10.21608/ejar.2011.177827
HASSAN, E., ABDEL WANIS, N., TOLBA, S. (2011). 'STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE', Egyptian Journal of Agricultural Research, 89(3), pp. 1101-1158. doi: 10.21608/ejar.2011.177827
HASSAN, E., ABDEL WANIS, N., TOLBA, S. STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE. Egyptian Journal of Agricultural Research, 2011; 89(3): 1101-1158. doi: 10.21608/ejar.2011.177827

STUDY OF INCORPORATION DEAE DEXTRAN DURING PRODUCTION OF LOCAL AVIAN INFLUENZA (AI) INACTIVATED VACCINE

Article 26, Volume 89, Issue 3, September 2011, Page 1101-1158  XML PDF (218.66 K)
Document Type: Original Article
DOI: 10.21608/ejar.2011.177827
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Authors
EMAN A. HASSAN; NABEL A. ABDEL WANIS; SUSAN K. TOLBA
Veterinary Serum and Vaccine Research Institute, Abbasia, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt.
Abstract
Two experiments have been conducted to study incorporation of di-ethyl amino ethyl dextran (DEAE Dextran) during production of avian influenza (AI) inactivated vaccine. The first was planned to study addition of DEAE Dextran with different concentrations (25g, 50g and 100g/0.1ml) to the virus inoculum seed before egg inoculation. Obtained results revealed selection of 25g DEAE Dextran/0.1 ml that resulted in increasing haemagglutinating activity (HA) with 2 log and virus titre (EID50) with 3.2. In the second experiment, the same concentrations were added and tested separately during vaccine preparation with two different adjuvants. Serum of vaccinated chickens with the vaccine to which 100 g DEAE Dextran / 0.5 ml was added, developed satisfactory raise in antibody level in comparing to positive control groups which received the vaccine without any additives.
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