FARGHALY, S., ABO-YOUSSEF, H., SENGAB, M., GHONEM, Y. (2010). IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS. Egyptian Journal of Agricultural Research, 88(1), 153-166. doi: 10.21608/ejar.2010.180255
SAUDA F. FARGHALY; HALA M. ABO-YOUSSEF; MOHAMED SENGAB; YAHYA F. GHONEM. "IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS". Egyptian Journal of Agricultural Research, 88, 1, 2010, 153-166. doi: 10.21608/ejar.2010.180255
FARGHALY, S., ABO-YOUSSEF, H., SENGAB, M., GHONEM, Y. (2010). 'IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS', Egyptian Journal of Agricultural Research, 88(1), pp. 153-166. doi: 10.21608/ejar.2010.180255
FARGHALY, S., ABO-YOUSSEF, H., SENGAB, M., GHONEM, Y. IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS. Egyptian Journal of Agricultural Research, 2010; 88(1): 153-166. doi: 10.21608/ejar.2010.180255
IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS
Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, Egypt.
Abstract
Mechanism of resistance to lambda-cyhalothrin in highly resistant Bemisia tabaci strain can be explained by the voltage-gated sodium channel which is the primary target site of pyrethroid insecticides. The super knockdown resistance (super-kdr) to pyrethroids is caused by changes at specific sites on the para-type sodium channel protein domain II (IIS4-6). The B. tabaci para-type sodium channel gene was RT-PCR amplified from lab-strain (reference), Parent (first generation after treatment with the insecticide) and generation thirteen after treatment was considered. The mechanisms of resistance to Lambda-cyhalothrin (Karat 20% EC) in a Q biotype, highly resistant Bemisia tabaci strain. Analysis of the sequence of the IIS4-IIS6 region of the para sodium channel gene of lab-strain, Parent and resistant strain (G13) revealed two amino acid replacements compared to that of the SUD-S susceptible strain. One is the leucine to isoleucine substitution at position 925 (L925I) and allele r1-Q1 (GenBank accession no. DQ205206) has identical intron sequences with samples of parent and lab-strain, other is a novel kdr resistant mutation for B. tabaci, a threonine to valine substitution at position (T929V) and alleles r2-Q1 (GenBank accession no DQ205207) has identical intron sequence with G13..
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