PREPARATION OF PEROXIDASE CONJUGATED AND NON- CONJUGATED ANTISHEEP IgM IMMUNOGLOBULIN

SAYED, TARADI A.; HASSAN, KAERM E. Z.; WARDA, SAFAA, A.; TAHA, MOHAMED M.; DAOUD, AHMED M.

doi:10.21608/ejar.2007.218399

PREPARATION OF PEROXIDASE CONJUGATED AND NON- CONJUGATED ANTISHEEP IgM IMMUNOGLOBULIN

Document Type : Original Article

Authors

¹ Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

² Central Laboratory for evaluation of Veterinary Biologics, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

10.21608/ejar.2007.218399

Abstract

Peroxidase conjugated and non-conjugated antisheep immuno-globulin-M (IgM) were prepared from rabbit hyperimmune serum. The optimal dilutions of both products were determined by checkerboard ELISA method which proved their efficacy. Their titres were 1:2000 and 1:100, respectively. Both peroxidase conjugated and non-conjugated antisheep IgM, in comparing with imported standardized product were applied on 28 serum samples collected from sheep infected with Rift Valley Fever virus (RVFV) to detect IgM antibodies against RVFV using both indirect ELISA and IgM capture ELISA. The results revealed that non-conjugated antisheep IgM using IgM capture ELISA was superior to peroxidase conjugated antisheep IgM using indirect ELISA for detection of IgM antibodies in sheep.