HASSAN, M. (2006). USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA. Egyptian Journal of Agricultural Research, 84(2), 311-321. doi: 10.21608/ejar.2006.230295
MAGGIE E. M. HASSAN. "USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA". Egyptian Journal of Agricultural Research, 84, 2, 2006, 311-321. doi: 10.21608/ejar.2006.230295
HASSAN, M. (2006). 'USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA', Egyptian Journal of Agricultural Research, 84(2), pp. 311-321. doi: 10.21608/ejar.2006.230295
HASSAN, M. USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA. Egyptian Journal of Agricultural Research, 2006; 84(2): 311-321. doi: 10.21608/ejar.2006.230295
USING RAPD-PCR TECHNIQUES IN IDENTIFICATION OF COTTON FUSARIA
Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Abstract
The development of wilt symptoms is the main criterion for the identification of such isolates A oxyspotum f. sp. vasinfectunz This biological test is time consuming and sensitive to temperature changes. Therefore, differentation between A axyspotum 1. sp. vasinfeaum isolates from the recently isolated damping-off producing isolates of A owsporum are being explored by RAPD-PCR using any of the primers d (CGTGCGGGAA)-3 or d (AAGAGCCCGT)-3 or d (AACGCGCAAC)-3 or d (CCCGTCAGCA)-3 by RAPD-PCR. The method was found sensitive, aozurate, practical and time saving in recognizing F arysporum f. sp. vasinfectum isolates, which showed a very high degree of similarity. In =bast, the damping-off causing F. oxysporum isolates tested showed different dusters with a low overall similarity.
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