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YOUSSEF, N., GABRY, G., HASSAN, M., SHEMIES, O. (2003). OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS. Egyptian Journal of Agricultural Research, 81(2), 871-791. doi: 10.21608/ejar.2003.278030
NAWAL M. A. YOUSSEF; GABER H. GABRY; MOHAMED H. HASSAN; OMAYMA A. A. SHEMIES. "OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS". Egyptian Journal of Agricultural Research, 81, 2, 2003, 871-791. doi: 10.21608/ejar.2003.278030
YOUSSEF, N., GABRY, G., HASSAN, M., SHEMIES, O. (2003). 'OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS', Egyptian Journal of Agricultural Research, 81(2), pp. 871-791. doi: 10.21608/ejar.2003.278030
YOUSSEF, N., GABRY, G., HASSAN, M., SHEMIES, O. OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS. Egyptian Journal of Agricultural Research, 2003; 81(2): 871-791. doi: 10.21608/ejar.2003.278030

OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS

Article 27, Volume 81, Issue 2, July 2003, Page 871-791  XML PDF (2.85 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2003.278030
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Authors
NAWAL M. A. YOUSSEF; GABER H. GABRY; MOHAMED H. HASSAN; OMAYMA A. A. SHEMIES
Animal Health Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
In a herd of 790 imported Djiboutian dromedary camels located at Suez quarantine station, respiratory signs accompanied by ocular dis‌charges and weakness were observed. PI-3 and BHV-I viruses were isolated on MDBK cell line from nasal and ocular swabs of diseased camels and from organs (lung, liver & spleen) of emergency slaughtered camels. Mixed infection with PI-3 and EtHV-I were recorded. PI-3 virus was identified by Haemagglutination Inhibition (HI) test with reference PI-3 antiserum and 81-IV-I by Indirect Fluorescent Anti‌body (IFA) technique using reference BHV-I antiserum. Agar Gel Precipitation Test (AGPT) and Dot-Enzyme Linked Im-mune Sorbent Assay (ELISA) were applied on the above-mentioned sam‌ples for rapid detection of PI-3 and 8HV-I viral antigens. Also. 86 serum samples were collected from apparently healthy and diseased camels, tested by Virus Neutralization Test (VNT) for de-tection of neutralizing antibodies against PI-3 and 8HV-I.. and by HI-test for PI-3 heam agglutinating antibodies. This is the first record for detection and isolation of PI-3 and BHV-I viruses (mixed infection) among camels in Egypt. 
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