OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS

YOUSSEF, NAWAL M. A.; GABRY, GABER H.; HASSAN, MOHAMED H.; SHEMIES, OMAYMA A. A.

doi:10.21608/ejar.2003.278030

OCCURRENCE OF PARAINFLUENZA TYPE -3 (PI-3) AND BOVINE HERPES VIRUS TYPE-I (BHV-I) VIRUSES (MIXED INFECTION) AMONG CAMELS

Document Type : Original Article

Authors

Animal Health Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt

10.21608/ejar.2003.278030

Abstract

In a herd of 790 imported Djiboutian dromedary camels located at Suez quarantine station, respiratory signs accompanied by ocular dis‌charges and weakness were observed. PI-3 and BHV-I viruses were isolated on MDBK cell line from nasal and ocular swabs of diseased camels and from organs (lung, liver & spleen) of emergency slaughtered camels. Mixed infection with PI-3 and EtHV-I were recorded. PI-3 virus was identified by Haemagglutination Inhibition (HI) test with reference PI-3 antiserum and 81-IV-I by Indirect Fluorescent Anti‌body (IFA) technique using reference BHV-I antiserum. Agar Gel Precipitation Test (AGPT) and Dot-Enzyme Linked Im-mune Sorbent Assay (ELISA) were applied on the above-mentioned sam‌ples for rapid detection of PI-3 and 8HV-I viral antigens. Also. 86 serum samples were collected from apparently healthy and diseased camels, tested by Virus Neutralization Test (VNT) for de-tection of neutralizing antibodies against PI-3 and 8HV-I.. and by HI-test for PI-3 heam agglutinating antibodies. This is the first record for detection and isolation of PI-3 and BHV-I viruses (mixed infection) among camels in Egypt.