EL-REFAII, M. (2003). TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE. Egyptian Journal of Agricultural Research, 81(2), 805-816. doi: 10.21608/ejar.2003.278043
MAGDA A. H. EL-REFAII. "TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE". Egyptian Journal of Agricultural Research, 81, 2, 2003, 805-816. doi: 10.21608/ejar.2003.278043
EL-REFAII, M. (2003). 'TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE', Egyptian Journal of Agricultural Research, 81(2), pp. 805-816. doi: 10.21608/ejar.2003.278043
EL-REFAII, M. TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE. Egyptian Journal of Agricultural Research, 2003; 81(2): 805-816. doi: 10.21608/ejar.2003.278043
TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE
Animal Health Research Institute, Agricultural Research Centre, Ministry of Agriculture, Giza, Egypt
Abstract
This study was carried out to determine the therapeutic effect of Nigelle saliva oil extract on mice experimentally infected with Cryptosporidium parvum. For this purpose. 25 susceptible swiss mice (2-3 weeks old) were divided into 3 groups. Group I included infected 11 treated mice, group II included 10 infected non-treated ones, and group III composed of 4 non-infected non-treated mice. One week before experimental infection, all mice were immunosupressed by a mixture of 2 mg/t. dexamethasone sodium phosphate and SOO mg/L tetracycline hydrochloride In daily fresh drinking water. Experimental infection of mice was carried out with cryptospoddium pawum oocysts isolated from naturally infected calves. One week later, mice of group I were orally treated with Nigelle oil at a dose of 1 m1/100g body weight for each mouse. Faecal pellets from rectum of each mouse in all groups were examined every other day post-treatment by the Modified Ziehl-Neelsen technique. 0e-cysts intensities were scored in 100 randomly selected fields. The results revealed that mice of group III were refractory, while, mice of group I showed a reduction in oocysts count when compared to those of group II. On day 35th, all mice were sacrificed and parts of their ilea were fixed in 10% formalin solution for histopathotogical examination, as well as, smears from ilea were taken and stained with the Modified Ziehl-Neelsen technique. Villi of ileum of mice in group I showed normal appearance without any changes, while, those of mice of group II showed apparent villus atrophy: shortening and blunting with desquamation of most of villi. Villi of group III were normal. Data were statistically analysed and discussed.
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