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HASSAN, M., KHALIL, M., FARAG, N., HARPHOUSH, S. (2003). DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA. Egyptian Journal of Agricultural Research, 81(4), 1505-1520. doi: 10.21608/ejar.2003.287181
MAGGIE E. M. HASSAN; MOHAMED S. KHALIL; NABIL S. FARAG; SHERIEN A. HARPHOUSH. "DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA". Egyptian Journal of Agricultural Research, 81, 4, 2003, 1505-1520. doi: 10.21608/ejar.2003.287181
HASSAN, M., KHALIL, M., FARAG, N., HARPHOUSH, S. (2003). 'DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA', Egyptian Journal of Agricultural Research, 81(4), pp. 1505-1520. doi: 10.21608/ejar.2003.287181
HASSAN, M., KHALIL, M., FARAG, N., HARPHOUSH, S. DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA. Egyptian Journal of Agricultural Research, 2003; 81(4): 1505-1520. doi: 10.21608/ejar.2003.287181

DIFFERENTIATION AMONG RALSTONIA SOLANACEARUM ISOLATES CAUSING POTATO BACTERIAL WILT BY RANDOM AMPLIFIED POLYMORPHIC DNA

Article 2, Volume 81, Issue 4, December 2003, Page 1505-1520  XML PDF (3.59 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2003.287181
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Authors
MAGGIE E. M. HASSAN; MOHAMED S. KHALIL; NABIL S. FARAG; SHERIEN A. HARPHOUSH
Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Abstract
Ten Ralstonia solanaceannn isolatos were obtained from Spunta and Nicola potato cultivars . PCR-based RAPD analysis was carried out, using four primers, on agarose and polyacrylamide gels as support media. Only, the primer 6-d (AACGCGCAAC)-3 differentiated the Isolates recov­ered from the two cultivars, others did not. In general, separation on poly-acrylamide and staining with silver nitrate gave better resolution than those on agarose and stained with ethidium bromide. Further inves­tigations are needed using greater number of :solates, from different po­tato cultivars and /or different sources, to confirm the result as well as the possible effect of the host cultivars on the properties of bacterial DNA.
Keywords
Ralstonia solanacearum; potato cultivars; polyacryl‌amide; silvor nitrate and RAPD
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