PRODUCTION AND EVALUATION OF A NEWLY ADJUVANTED RABBIT PASTEURELLOSIS VACCINE

DAOUD, AHMED M.; MAHMOUD, MANAL S.; FADL, HALA A.; YOUSSEF, ELHAM A.; HASSAN, HASSAN A.

doi:10.21608/ejar.2003.298972

PRODUCTION AND EVALUATION OF A NEWLY ADJUVANTED RABBIT PASTEURELLOSIS VACCINE

Document Type : Original Article

Authors

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

10.21608/ejar.2003.298972

Abstract

Rabbit pasteurellosis aluminium hydroxide gel adjuvanted vaccine (ALV) and aqueous formalized one (AV) were evaluated serologically and by 2-stage protection bioassay. Evaluation in mice revealed a good protection level (85%) in either ALV or AV vaccinates, when subjected to homogenous challenge exposure. In rabbits, both vaccines stimulated satisfactory and significantly differed antibody levels. However, level recorded in ALV vaccinates persisted for more prolonged period (16^th week post-boostering, PB) than in case of AV. Moreover, sera from rabbits immunized with ALV or AV form passively protected mice at a levels of 70% and 60%, respectively. The l^et-stage bioassay conducted at rⁱ weeks PB revealed no difference in the level of protective immunity (80%) between rabbits vaccinated with either forms of vaccines. However, the 2^m-stage bioassay conducted at 16^th week PB revealed a superior protective level (70%) in ALV vaccinates versus 50% only in AV ones. The ALV-based Pasteur°Ua vaccine proved to be safe and effective alternative to the classical aqueous one for controlling P. multocida infection in rabbits.