TRIAL FOR PRODUCTION OF DIAGNOSTIC KIT FOR DIAGNOSIS OF EQUINE VIRAL DISEASES

Document Type : Original Article

Authors

Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt

Abstract

A trial for production of diagnostic kit for diagnosis of equine viral disease was done. The antibodies were raised in white New-Zealand rab­bits. The conjugation was achieved through application of the most pop­ular, easier and cheaper method (Periodate method). The optimal dilu­tion of the conjugate was determined by using of known AHSV antigen together with negative one. The conjugate was used in solid phase ELISA on 50 horse sera.