ESSMAIL, M., BAKER, D., VANDEWOUDE, S., COLLINS, J., SALMAN, M., HEGAZY, A., WAHBAH, M. (2001). EXPERIMENTAL INFECTION OF BUCKS AND PREGNANT DOES WITH BOVINE HERPESVIRUS-4. Egyptian Journal of Agricultural Research, 79(4), 1605-1620. doi: 10.21608/ejar.2001.320959
MOHAMED E. ESSMAIL; DELL C. BAKER; SOE VANDEWOUDE; JAMES COLLINS; MOWAFK SALMAN; ALI A. HEGAZY; MOHAMED A. WAHBAH. "EXPERIMENTAL INFECTION OF BUCKS AND PREGNANT DOES WITH BOVINE HERPESVIRUS-4". Egyptian Journal of Agricultural Research, 79, 4, 2001, 1605-1620. doi: 10.21608/ejar.2001.320959
ESSMAIL, M., BAKER, D., VANDEWOUDE, S., COLLINS, J., SALMAN, M., HEGAZY, A., WAHBAH, M. (2001). 'EXPERIMENTAL INFECTION OF BUCKS AND PREGNANT DOES WITH BOVINE HERPESVIRUS-4', Egyptian Journal of Agricultural Research, 79(4), pp. 1605-1620. doi: 10.21608/ejar.2001.320959
ESSMAIL, M., BAKER, D., VANDEWOUDE, S., COLLINS, J., SALMAN, M., HEGAZY, A., WAHBAH, M. EXPERIMENTAL INFECTION OF BUCKS AND PREGNANT DOES WITH BOVINE HERPESVIRUS-4. Egyptian Journal of Agricultural Research, 2001; 79(4): 1605-1620. doi: 10.21608/ejar.2001.320959
EXPERIMENTAL INFECTION OF BUCKS AND PREGNANT DOES WITH BOVINE HERPESVIRUS-4
1Animal Reproduction Research Institute, Agricultural Research Centre, Ministry of Agricultural, Giza, Egypt
2College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO., USA.
3Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Abstract
Fifty-six does were inoculated with bovine herpesvirus-4 (BHV-4) or normal cell culture fluid by intravaginal (groups 1 & 2, early gestation) or intravenous routes (groups 3 & 4, late gestation). Forty bucks were inoculated with BHV-4 or normal cell culture fluid by intratesticular (groups 5 & 6) or intravenous (groups 7 & 8) routes. Clinical signs following inoculation of the virus in does included abortion in 2 pregnant does, decreased conception rate (25% conception rate in group 1 vs. 41.66% conception rate in control group 2), and mild vaginitis (2124, group 1). No clinical signs were observed with viral infection in bucks. Microscopic lesions included marked placentilis, mild suppurative end*. metritis, moderate multifocal endometrial hemorrhages, endometrial oedema, oophoritis, mesenteric lymph node hyperplasia, and mild multifocal necrotizing hepatitis. Histopathological examination of intratesticularly inoculated rabbits revealed mild to moderate interstitial lymphocytic and acute necrotizing orchitis. with segmental to diffuse testicular degeneration, and mild interstitial oedema. The virus was isolated from one testicle of inoculated rabbit. Viral antigen was detected by immunohistochemistry only in the mesenteric lymph nodes and spleen in infected does, but virus was not isolated. Polymerase chain reaction (PCR) detected BHV-4 genome in two bucks. Transmission electron microscopy (TEM) revealed viral nucleocapsids in the uterus, vagina, spleen, and mesenteric lymph nodes of infected does and in the testis, spleen and mesenteric lymph node of infected bucks. Gross, microscopic, TEM, PCR, and immunohistochemical examination of tissues suggested that BHV-4 had a mild pathogenic role in bucks inoculated intratesticularly.
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