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MOHAMED, G., WASSEL, M., ELIAN, K., ZAKI, F. (2001). PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN. Egyptian Journal of Agricultural Research, 79(4), 1621-1631. doi: 10.21608/ejar.2001.320965
GEHAN K. MOHAMED; MOHAMED S. WASSEL; KHIRAT A. ELIAN; FARED F. ZAKI. "PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN". Egyptian Journal of Agricultural Research, 79, 4, 2001, 1621-1631. doi: 10.21608/ejar.2001.320965
MOHAMED, G., WASSEL, M., ELIAN, K., ZAKI, F. (2001). 'PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN', Egyptian Journal of Agricultural Research, 79(4), pp. 1621-1631. doi: 10.21608/ejar.2001.320965
MOHAMED, G., WASSEL, M., ELIAN, K., ZAKI, F. PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN. Egyptian Journal of Agricultural Research, 2001; 79(4): 1621-1631. doi: 10.21608/ejar.2001.320965

PRODUCTION AND EVALUATION OF ANTI RIFT VALLEY FEVER (RVF) IgG CONJUGATED WITH FLUORESCEIN

Article 26, Volume 79, Issue 4, October 2001, Page 1621-1631  XML PDF (2.44 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2001.320965
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Authors
GEHAN K. MOHAMED; MOHAMED S. WASSEL; KHIRAT A. ELIAN; FARED F. ZAKI
Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt
Abstract
Three susceptible sheep were inoculated with RVF antigen mixed with incomplete and complete Freund's adjuvant and without adjuvant in three successive doses. The hyperimmune sera collected from the sheep were tested against standard reference RVF antigen for the presence of specific RVF antibodies using serum neutralization test (SNT), agar gel precipitation test (AGPT) and indirect fluorescent assay (IFA). Separa­tion of RVF immunoglobulins were done using affinity chromatography (activated sepharose 46) followed by conjugation of RVF IgG with fluo­rescein isothiocyanale at p1-19.6. The conjugatedrRVF antibodies were ti­trated against reference RVF antigen and the titre reached (1:128). The working dilution was used for evaluation of different batches of attenu­ated and inactivated RVF vaccines in comparison with IFA test using anti-sheep Fitic, the results of both assays proved to be similar.
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