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ZEIDAN, S., TAHA, M., KASSEM, I. (2000). PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 78(3), 1337-1349. doi: 10.21608/ejar.2000.323909
SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM. "PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS". Egyptian Journal of Agricultural Research, 78, 3, 2000, 1337-1349. doi: 10.21608/ejar.2000.323909
ZEIDAN, S., TAHA, M., KASSEM, I. (2000). 'PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS', Egyptian Journal of Agricultural Research, 78(3), pp. 1337-1349. doi: 10.21608/ejar.2000.323909
ZEIDAN, S., TAHA, M., KASSEM, I. PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 2000; 78(3): 1337-1349. doi: 10.21608/ejar.2000.323909

PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS

Article 29, Volume 78, Issue 3, September 2000, Page 1337-1349  XML PDF (3.21 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2000.323909
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Authors
SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM
Serum and Vaccine Production Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
A preliminary study was conducted in which polycolonal antiser­um against bovine viral diarrhoea virus (BVDV), was prepared in New­zeland white rabbit. The antibodies were titrated by both serum neutrali­zation test (SNT) and enzyme linked immunosorbent assay (ELISA) techniques. The immunoglobuline was precipitated from the serum by con­centrated ammonium sulphate solution and was conjugated with fluores­cine isothiocyanate (FITC). Both non-reacted FITC and heavily FITC con­jugated immunoglobuline were removed. The optimal dilution of the conjugate and its specificity were determined by using BVDV infected tissue cultures. Seventy-five bovine tissue specimens collected from Cairo abat­toir were used for BVDV isolation, as well as for direct fluorescence anti­body (FA) techniques and their results were compared.
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