A preliminary study was conducted in which polycolonal antiserum against bovine viral diarrhoea virus (BVDV), was prepared in Newzeland white rabbit. The antibodies were titrated by both serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA) techniques. The immunoglobuline was precipitated from the serum by concentrated ammonium sulphate solution and was conjugated with fluorescine isothiocyanate (FITC). Both non-reacted FITC and heavily FITC conjugated immunoglobuline were removed. The optimal dilution of the conjugate and its specificity were determined by using BVDV infected tissue cultures. Seventy-five bovine tissue specimens collected from Cairo abattoir were used for BVDV isolation, as well as for direct fluorescence antibody (FA) techniques and their results were compared.
ZEIDAN, S. M., TAHA, M. M., & KASSEM, I. K. A. (2000). PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 78(3), 1337-1349. doi: 10.21608/ejar.2000.323909
MLA
SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM. "PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS", Egyptian Journal of Agricultural Research, 78, 3, 2000, 1337-1349. doi: 10.21608/ejar.2000.323909
HARVARD
ZEIDAN, S. M., TAHA, M. M., KASSEM, I. K. A. (2000). 'PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS', Egyptian Journal of Agricultural Research, 78(3), pp. 1337-1349. doi: 10.21608/ejar.2000.323909
VANCOUVER
ZEIDAN, S. M., TAHA, M. M., KASSEM, I. K. A. PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 2000; 78(3): 1337-1349. doi: 10.21608/ejar.2000.323909
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