Home Articles List Article Information
Egyptian Journal of Agricultural Research
Journal Archive
Volume Volume 103 (2025)
Volume Volume 102 (2024)
Volume Volume 101 (2023)
Volume Volume 100 (2022)
Volume Volume 99 (2021)
Volume Volume 98 (2020)
Volume Volume 97 (2019)
Volume Volume 96 (2018)
Volume Volume 95 (2017)
Volume Volume 94 (2016)
Volume Volume 93 (2015)
Volume Volume 92 (2014)
Volume Volume 91 (2013)
Volume Volume 90 (2012)
Volume Volume 89 (2011)
Volume Volume 88 (2010)
Volume Volume 87 (2009)
Volume Volume 86 (2008)
Volume Volume 85 (2007)
Volume Volume 84 (2006)
Volume Volume 83 (2005)
Volume Volume 82 (2004)
Volume Volume 81 (2003)
Volume Volume 80 (2002)
Volume Volume 79 (2001)
Volume Volume 78 (2000)
Volume Volume 77 (1999)
Volume Volume 76 (1998)
Volume Volume 75 (1997)
Volume Volume 74 (1996)
ZEIDAN, S., TAHA, M., KASSEM, I. (2000). PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 78(3), 1337-1349. doi: 10.21608/ejar.2000.323909
SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM. "PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS". Egyptian Journal of Agricultural Research, 78, 3, 2000, 1337-1349. doi: 10.21608/ejar.2000.323909
ZEIDAN, S., TAHA, M., KASSEM, I. (2000). 'PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS', Egyptian Journal of Agricultural Research, 78(3), pp. 1337-1349. doi: 10.21608/ejar.2000.323909
ZEIDAN, S., TAHA, M., KASSEM, I. PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS. Egyptian Journal of Agricultural Research, 2000; 78(3): 1337-1349. doi: 10.21608/ejar.2000.323909

PRODUCTION OF SPECIFIC IMMUNOFLUORESCENT HYPERIMMUNE SERUM FOR DIAGNOSIS OF BOVINE DIARRHOEA VIRUS

Article 29, Volume 78, Issue 3, September 2000, Page 1337-1349  XML PDF (3.21 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2000.323909
View on SCiNiTO View on SCiNiTO
Authors
SAYED M. ZEIDAN; MOHAMED M. TAHA; IMAN K. A. KASSEM
Serum and Vaccine Production Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
A preliminary study was conducted in which polycolonal antiser­um against bovine viral diarrhoea virus (BVDV), was prepared in New­zeland white rabbit. The antibodies were titrated by both serum neutrali­zation test (SNT) and enzyme linked immunosorbent assay (ELISA) techniques. The immunoglobuline was precipitated from the serum by con­centrated ammonium sulphate solution and was conjugated with fluores­cine isothiocyanate (FITC). Both non-reacted FITC and heavily FITC con­jugated immunoglobuline were removed. The optimal dilution of the conjugate and its specificity were determined by using BVDV infected tissue cultures. Seventy-five bovine tissue specimens collected from Cairo abat­toir were used for BVDV isolation, as well as for direct fluorescence anti­body (FA) techniques and their results were compared.
Statistics
Article View: 43
PDF Download: 197