SHAFEY, S., EI-EBIARY, E., GERGIS, S. (1999). COMPARISON OF DIFFERENT MEDIA AND ENVIRONMENTAL CULTURAL CONDITION ON THE YIELD OF HAEMOPH1LUS PARAGALLINARUM STRAIN. Egyptian Journal of Agricultural Research, 77(2), 971-981. doi: 10.21608/ejar.1999.336286
SAMY M. SHAFEY; ELHAM A. EI-EBIARY; SAHER M. GERGIS. "COMPARISON OF DIFFERENT MEDIA AND ENVIRONMENTAL CULTURAL CONDITION ON THE YIELD OF HAEMOPH1LUS PARAGALLINARUM STRAIN". Egyptian Journal of Agricultural Research, 77, 2, 1999, 971-981. doi: 10.21608/ejar.1999.336286
SHAFEY, S., EI-EBIARY, E., GERGIS, S. (1999). 'COMPARISON OF DIFFERENT MEDIA AND ENVIRONMENTAL CULTURAL CONDITION ON THE YIELD OF HAEMOPH1LUS PARAGALLINARUM STRAIN', Egyptian Journal of Agricultural Research, 77(2), pp. 971-981. doi: 10.21608/ejar.1999.336286
SHAFEY, S., EI-EBIARY, E., GERGIS, S. COMPARISON OF DIFFERENT MEDIA AND ENVIRONMENTAL CULTURAL CONDITION ON THE YIELD OF HAEMOPH1LUS PARAGALLINARUM STRAIN. Egyptian Journal of Agricultural Research, 1999; 77(2): 971-981. doi: 10.21608/ejar.1999.336286
COMPARISON OF DIFFERENT MEDIA AND ENVIRONMENTAL CULTURAL CONDITION ON THE YIELD OF HAEMOPH1LUS PARAGALLINARUM STRAIN
1Central Laboratory for control of Veterinary Biologics, Agricultural Research Centre, Giza, Egypt
2Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Giza, Egypt
Abstract
This study revealed that the best cultural conditions to produce a high yield of H.paragallinarum (HP) bacterial cells for bacterin or antigen preparation was the fermentative method. The best yield on dry weight basis was obtained by chicken meat infusion broth 'CM" with 0.5% chicken serum and brain heart infusion broth "BHI' with 1% horse serum after spreger 10% CO2 gassing in the media for four hours and incubation at 37°C for 18 hours. Cultures produced by this method and diluted twice produce an effective protective bacterin and sensitive specific antigen. This finding has an economical value.
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