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Egyptian Journal of Agricultural Research
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EL-EBIARY, E., SHAFAY, S. (1999). COMPARISON BETWEEN MYCOPLASMA GALLISEPTICUM F. STRAIN AND LOCAL FIELD ISOLATES BY USING SOS-PAGE AND WESTERN BLOT ANALYSIS. Egyptian Journal of Agricultural Research, 77(3), 1399-1413. doi: 10.21608/ejar.1999.337764
ELHAM A. EL-EBIARY; SAMY M. SHAFAY. "COMPARISON BETWEEN MYCOPLASMA GALLISEPTICUM F. STRAIN AND LOCAL FIELD ISOLATES BY USING SOS-PAGE AND WESTERN BLOT ANALYSIS". Egyptian Journal of Agricultural Research, 77, 3, 1999, 1399-1413. doi: 10.21608/ejar.1999.337764
EL-EBIARY, E., SHAFAY, S. (1999). 'COMPARISON BETWEEN MYCOPLASMA GALLISEPTICUM F. STRAIN AND LOCAL FIELD ISOLATES BY USING SOS-PAGE AND WESTERN BLOT ANALYSIS', Egyptian Journal of Agricultural Research, 77(3), pp. 1399-1413. doi: 10.21608/ejar.1999.337764
EL-EBIARY, E., SHAFAY, S. COMPARISON BETWEEN MYCOPLASMA GALLISEPTICUM F. STRAIN AND LOCAL FIELD ISOLATES BY USING SOS-PAGE AND WESTERN BLOT ANALYSIS. Egyptian Journal of Agricultural Research, 1999; 77(3): 1399-1413. doi: 10.21608/ejar.1999.337764

COMPARISON BETWEEN MYCOPLASMA GALLISEPTICUM F. STRAIN AND LOCAL FIELD ISOLATES BY USING SOS-PAGE AND WESTERN BLOT ANALYSIS

Article 34, Volume 77, Issue 3, September 1999, Page 1399-1413  XML PDF (3.88 MB)
Document Type: Original Article
DOI: 10.21608/ejar.1999.337764
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Authors
ELHAM A. EL-EBIARY; SAMY M. SHAFAY
Central Laboratory for control of Veterinary Biologics, Agricultural Research Cen‌tre, Egypt
Abstract
Various strains of M.gallisepticum (MG) including F-strain (as a reference) were examined by SAS-PAGE. Minor but distinct and repro­duced differences in protein banding patterns were detected between strains. Cross reactivities among MG strains were examined by using rab­bit polyclonal antisera. The major antigens and cross reacting epitopes of the MG strains were demonstrated by immunoblotting assay using ho­mologous antisera.
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