MOHAMMED, K., ISMAIL, S. (1999). APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS. Egyptian Journal of Agricultural Research, 77(3), 1415-1431. doi: 10.21608/ejar.1999.337765
KAMEL M. E. MOHAMMED; SAYED T. ISMAIL. "APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS". Egyptian Journal of Agricultural Research, 77, 3, 1999, 1415-1431. doi: 10.21608/ejar.1999.337765
MOHAMMED, K., ISMAIL, S. (1999). 'APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS', Egyptian Journal of Agricultural Research, 77(3), pp. 1415-1431. doi: 10.21608/ejar.1999.337765
MOHAMMED, K., ISMAIL, S. APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS. Egyptian Journal of Agricultural Research, 1999; 77(3): 1415-1431. doi: 10.21608/ejar.1999.337765
APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS
1Animal Reproduction Research Institute. Agricultural Research Centre, Dokki, Giza, Egypt
2Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Abstract
Different regimens of superovulation were applied to 33 non-lactating Friesian cows for embryo production. These animals were randomly classifed into 6 groups of superovulatory treatments. Group 1 (n=5) was injected IM with a single dose of 2000 IU pregnant mare serum gonadotropins (PMSG) on day 10-12 of the estrous cycle. Luteolysis was induced by 2 injections of PGF2a (12 h interval) two days after PMSG treatment. The animal that showed signs of estrus was inseminated. Flushing was carried out non-surgically on day 7-9 of the estrus. Group 2 (n=5) and group 3 (n = 7) were treated as group 1, but the dose of PMSG was 2500 IU (Group 2) and 3000 10 (Group 3). The animals of Group 4 (n = 7) were primed with 200 IU/PMSG on day 5 and received 2800 IU/PMSG on day 10 of the cycle. All animals of this group were injected with 2 doses of PGF2a; 48 h after second dose of PMSG. Group 5 (n = 5) and 6 (n = 4) were treated as Group 4, but the priming doses of PMSG were 500 and 1000 IU/PMSG and the main doses of PMSG injected on day 10 were 2500 and 2000 IU/PMSG for Group 5 and 6, respectively. The mean values of CL were 7.6±1.36, 12.8±1.06, 9.57±1.17, 16.0±1.75, 13.8±0.96 and 5.57±0.85, for follicles, it was 1.8±0.66,2.6±0.40, 3.0±0.31, 4.57±0.81, 4.2-10.37 and 2.5±0.29, recovered embryos ova, it was 5.4±1.03, 8.6±0.60, 5.17±0.70, 7.07±1.06, 6.4±0.92 and 2.25 ± 0.85, for fertilized ova, it was 5.0±0.71, 7.0±0.54, 4.67±0.67, 6.5±0.85, 5.0±0.83 and 2.0±0.82, for transferable embryo, it was 4.2±0.79, 6.0±0.71, 3.8±0.54, 5.67±89, 4.4±0.67 and 1.75±0.85, for groups 1,2,3,4,5 and 6, respectively. The best results, as indicated by total ovarian response, CL, recovered embryo/ova, fertilized ova and transferable embryos were obtained in Group 4, when cows primed with 200 IU/PMSG early on Day 5 and 2800 IL•/PMSG on Day 10 of the estrous cycle. However, the finding results showed that, regimens of superovulatory treatment had a significant effect on the ovarian response and embryo recovery, and no significant effect on the embryo quality. Calving rates after transfer of single embryo were 44.1% and 42.9% for morula and blastocyst stages, respectively, with no significant differences between the 2 stages, and the overall mean was 43.6%, while, the calving rate after transfer double embryos was 47.6%. The grade cf embryos had a significant (P<0.06) effect on the calving rate, it was 53.57 and 33.33% for grade I and II embryos, respectively.
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