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ABBAS, H., YOUSSEF, R., LABIB, F., SELMI, A., MANSOUR, H. (1999). FACTORS AFFECTING IN-VITRO FERTILIZATION OF EGYPTIAN BUFFALOES WITH SPECIAL REFFERENCE TO SPERM-ZONA PELLUCIDA BINDING ASSAY. Egyptian Journal of Agricultural Research, 77(4), 1815-1826. doi: 10.21608/ejar.1999.343048
HISHAM E. ABBAS; RAOF H. YOUSSEF; FATEN M. LABIB; ATEF A. SELMI; HASSAN A. MANSOUR. "FACTORS AFFECTING IN-VITRO FERTILIZATION OF EGYPTIAN BUFFALOES WITH SPECIAL REFFERENCE TO SPERM-ZONA PELLUCIDA BINDING ASSAY". Egyptian Journal of Agricultural Research, 77, 4, 1999, 1815-1826. doi: 10.21608/ejar.1999.343048
ABBAS, H., YOUSSEF, R., LABIB, F., SELMI, A., MANSOUR, H. (1999). 'FACTORS AFFECTING IN-VITRO FERTILIZATION OF EGYPTIAN BUFFALOES WITH SPECIAL REFFERENCE TO SPERM-ZONA PELLUCIDA BINDING ASSAY', Egyptian Journal of Agricultural Research, 77(4), pp. 1815-1826. doi: 10.21608/ejar.1999.343048
ABBAS, H., YOUSSEF, R., LABIB, F., SELMI, A., MANSOUR, H. FACTORS AFFECTING IN-VITRO FERTILIZATION OF EGYPTIAN BUFFALOES WITH SPECIAL REFFERENCE TO SPERM-ZONA PELLUCIDA BINDING ASSAY. Egyptian Journal of Agricultural Research, 1999; 77(4): 1815-1826. doi: 10.21608/ejar.1999.343048

FACTORS AFFECTING IN-VITRO FERTILIZATION OF EGYPTIAN BUFFALOES WITH SPECIAL REFFERENCE TO SPERM-ZONA PELLUCIDA BINDING ASSAY

Article 32, Volume 77, Issue 4, December 1999, Page 1815-1826  XML PDF (3.15 MB)
Document Type: Original Article
DOI: 10.21608/ejar.1999.343048
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Authors
HISHAM E. ABBAS1; RAOF H. YOUSSEF1; FATEN M. LABIB1; ATEF A. SELMI2; HASSAN A. MANSOUR2
1Animal Health Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt
2Faculty of Veterinary Medicine, Zagzig University, Egypt
Abstract
Assays based on spem-zona pellucida binding have been developing as diagnostic tests to predict the fertilizq/ion potential of bull spermatozoa. in this study a similar assay for buffalo sperm was used. The results showed a significant variation (P<0.01) in accessory sperm number between the fresh semen of three bulls, in spite of the same physical characters. Also three bulls, in their physical characters sand number of accessory sperm. The overall mean of accessory sperm was significantly increased (P<0.01) by fresh semen than by frozen semen (181.0 ± 3.0 Vs 30.7 ±7 ± 2.2). The effect of (2mM) caffeie and/or (10 pg/m1) heparin on the number of accessory sperm was significantly obvious (P<0.01) when compared with the control, while, there was no significant variations between them. Addition of both caffeine and heparin in the incubation media improved the number of accessory sperm than in fertilization media, but without significant variations. The number of the accessory sperm was insigificantly increased when the oocytes co-cultured in sperm droplets than when the sperm co-cultured with oocytes in fertilization medium.
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