TAHA, S., EL-SABBAGH, M., GHALY, H., ABDEL-SAMAE, M. (2001). PRODUCTION AND EVALUATION OF A MULTICOMPONENT INACTIVATED RESPIRATORY VIRAL VACCINE (BVD, IBR, PI-3 AND BOVINE ADENO VIRUS TYPE 3 (PNEUMO-4) IN CATTLE. Egyptian Journal of Agricultural Research, 79(1), 335-351. doi: 10.21608/ejar.2001.397693
SAMIRA S. TAHA; MAGDY M. A. EL-SABBAGH; HOSSUN M. GHALY; MOHAMED M. ABDEL-SAMAE. "PRODUCTION AND EVALUATION OF A MULTICOMPONENT INACTIVATED RESPIRATORY VIRAL VACCINE (BVD, IBR, PI-3 AND BOVINE ADENO VIRUS TYPE 3 (PNEUMO-4) IN CATTLE". Egyptian Journal of Agricultural Research, 79, 1, 2001, 335-351. doi: 10.21608/ejar.2001.397693
TAHA, S., EL-SABBAGH, M., GHALY, H., ABDEL-SAMAE, M. (2001). 'PRODUCTION AND EVALUATION OF A MULTICOMPONENT INACTIVATED RESPIRATORY VIRAL VACCINE (BVD, IBR, PI-3 AND BOVINE ADENO VIRUS TYPE 3 (PNEUMO-4) IN CATTLE', Egyptian Journal of Agricultural Research, 79(1), pp. 335-351. doi: 10.21608/ejar.2001.397693
TAHA, S., EL-SABBAGH, M., GHALY, H., ABDEL-SAMAE, M. PRODUCTION AND EVALUATION OF A MULTICOMPONENT INACTIVATED RESPIRATORY VIRAL VACCINE (BVD, IBR, PI-3 AND BOVINE ADENO VIRUS TYPE 3 (PNEUMO-4) IN CATTLE. Egyptian Journal of Agricultural Research, 2001; 79(1): 335-351. doi: 10.21608/ejar.2001.397693
PRODUCTION AND EVALUATION OF A MULTICOMPONENT INACTIVATED RESPIRATORY VIRAL VACCINE (BVD, IBR, PI-3 AND BOVINE ADENO VIRUS TYPE 3 (PNEUMO-4) IN CATTLE
1Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
2Faculty of Veterinary Medicine, Suez Canal University, Egypt
Abstract
An inactivated virus vaccine (MDBK-dextran) containing strains of bovine viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), parainfluenza type 3 (PI-3) and bovine adeno virus type-3 was conveniently produced by propagation on MDBK-dextran-roller system, then, the viruses were inactivated with binary ethyleneimine (BEI), lower concentration of inactivants which killed the viruses in the shortest period of time was selected for preparing the corresponding vaccine. Alhydrogel was added in a final concentration of 30% to the vaccine as adjuvant. Effectiveness of each fraction of the prepared vaccine was studied in challenged immunity studies. Cattle vaccinated with any fraction of vaccine followed by challenge with a disease causing strain of that fraction, resisted infection well by both forms; qualitative (clinical aspect) and quantitative points of view (virus isolation and temperature). All antigens stimulated high antibody titres detected by ELISA immuno assay (DA) and serum neutralization test (SNT) against all component of the vaccine and haemagglutination inhibition test (HIT) aganist P1-3. Immunity obtained was long-lasting and even persisted up to 6 months post-vaccination.
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