Home Articles List Article Information
Egyptian Journal of Agricultural Research
Journal Archive
Volume Volume 103 (2025)
Volume Volume 102 (2024)
Volume Volume 101 (2023)
Volume Volume 100 (2022)
Volume Volume 99 (2021)
Volume Volume 98 (2020)
Volume Volume 97 (2019)
Volume Volume 96 (2018)
Volume Volume 95 (2017)
Volume Volume 94 (2016)
Volume Volume 93 (2015)
Volume Volume 92 (2014)
Volume Volume 91 (2013)
Volume Volume 90 (2012)
Volume Volume 89 (2011)
Volume Volume 88 (2010)
Volume Volume 87 (2009)
Volume Volume 86 (2008)
Volume Volume 85 (2007)
Volume Volume 84 (2006)
Volume Volume 83 (2005)
Volume Volume 82 (2004)
Volume Volume 81 (2003)
Volume Volume 80 (2002)
Volume Volume 79 (2001)
Volume Volume 78 (2000)
Volume Volume 77 (1999)
Volume Volume 76 (1998)
Volume Volume 75 (1997)
TAHA, S. (2001). INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS. Egyptian Journal of Agricultural Research, 79(1), 361-372. doi: 10.21608/ejar.2001.397699
SAMIRA S. TAHA. "INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS". Egyptian Journal of Agricultural Research, 79, 1, 2001, 361-372. doi: 10.21608/ejar.2001.397699
TAHA, S. (2001). 'INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS', Egyptian Journal of Agricultural Research, 79(1), pp. 361-372. doi: 10.21608/ejar.2001.397699
TAHA, S. INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS. Egyptian Journal of Agricultural Research, 2001; 79(1): 361-372. doi: 10.21608/ejar.2001.397699

INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS

Article 28, Volume 79, Issue 1, March 2001, Page 361-372  XML PDF (4.34 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2001.397699
View on SCiNiTO View on SCiNiTO
Author
SAMIRA S. TAHA
Veterinary Serum and Vaccine Research institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
Infectivity titre and haemagglutination titre of PI-3 virus in MDBK cells using monolayer, suspension and roller systems revealed that the roller system produced the highest infectivity and haemagglutination in-hibition titre with an increase of loge 08 than their corresponding mono-layer cell culture. On the other hand, the infectivity and HA titres in cell suspension cultures were 1.0 logs 0 lower than their corresponding mon-olayer cell culture system. Influence of DEAE-Dextran for the enhancement of P1-3 virus in-fectivity in different cell culture systems, 25ug of DEAE-Dextran per ml is considered the safest concentration to the MDBK and VERO cell lines and giving these results: 1.Infectivity titre of PI-3 virus was markedly increased under the effect of both serial passages, and showed an increase of 1.5 logs 0 and 1.3 loge 0, respectively, more than their respective non-treated passages. 2.In the roller system, DEAE-Dextran enhanced the growth of virus by about 1.0 logi 0 and 1.2 logi 0 more than growth of untreated virus in both MDBK and VERO cell lines, respectively. 3.The highest titre obtained when using DEAE-Dextran MDBK roller sys-tem was 9.5 logs o.
Statistics
Article View: 18
PDF Download: 68