EFFECT OF SOME PESTICIDES ON THE SARCOPLASMIC PROTEIN FRACTIONATION OF COMMON CARP (CYPRINUS CARPI

RIZKALLA, ESSAM H.; EL-GAMAL, ABDEL-RAHMAN A.; MAHMOUD, AHMED A.; SHAWKY, AHMED S.; RAMADAN, AHMED A.

doi:10.21608/ejar.1997.403203

EFFECT OF SOME PESTICIDES ON THE SARCOPLASMIC PROTEIN FRACTIONATION OF COMMON CARP (CYPRINUS CARPI

Document Type : Original Article

Authors

¹ Animal Health Research Institute, Agricultural Research Centre, Giza, Egypt

² Central Laboratory for Aquaculture Research, Agricultural Research Centre, Dokki, Giza, Egypt

³ Faculty of Veterinary Medicine, Zagazig University, Egypt

10.21608/ejar.1997.403203

Abstract

Studying the genotoxic effects of different doses (1/10, 1/5 and 1/2 LC50) of organophosphorus compound (Hinosan) and carbamate (Sevin) for 30 days on the electropherograms of sarcoplasmic proteins of common carp (Cyprinus carpio) with regard to the number, mobility and density of fractions revactions that : 1.Ten percent polyacrylarnide gel electrophoresis of proteins extracted from untreated normal specimens yielded 14 bands. These bands were to be : the fastest mobile band, 4 distal bands, 5 mid bands, 3 proximal bands and the least mobile band. Fractions 1 and 4a showed the highest and lowest density percentage of all bands, respectively. 2.Hinosan exposed specimens yielded 13-15 bands. The percentage of appearance (Polymorphism) ranged from 33.3 - 100 %. A new band 8b appeared with 33.3 % polymorphism at 1/10 LC5 0 concentration. In addition, band 4b only appeared in 1/2 LC50 dose with 50% polymorphim. On the other hand, at 1/5 and 1/2 LC50 band 10 disappeared completely. 3.The differences in number of protein bands were marked in sevin treated specimens. 13-16 fractions were scannered with the three doses studied. At 1/2 LC50 dose, fractions 4b and 8b appeared newly. On the other hand, fractions 6 and 10 completely disappeared at both 1/10 and 1/5 LC50 doses. Percentage of polymorphism ranged from 33.3-100 %. 4.Comparison of the relative mobilities of bands between control and exposed Hinosan and Sevin specimens showed some amount of variations specially in the proximal fractions. 5.Studying the effects of to different concentrations of Hinosan and Sevin on changes in the relative proportions of protein fractions detected that, band 9 was the most strengthen fractions appeared in the current investigation. However, total distal fractions recorded a significant variation when fish were exposed to both pesticides.