SHAARAWY, M., Jen, H., SHWANG, X., SETHER, D. (1997). REACTIVITY OF SOME HAWAIIAN BANANA BUNCHY TOP DISEASED SAMPLES USING DAS-ELISA AND A DIGOXIGENIN LABELLED DNA PROBE. Egyptian Journal of Agricultural Research, 75(3), 515-527. doi: 10.21608/ejar.1997.406296
MOHAMED A. SHAARAWY; HU Jen; XIE W. SHWANG; DIANE SETHER. "REACTIVITY OF SOME HAWAIIAN BANANA BUNCHY TOP DISEASED SAMPLES USING DAS-ELISA AND A DIGOXIGENIN LABELLED DNA PROBE". Egyptian Journal of Agricultural Research, 75, 3, 1997, 515-527. doi: 10.21608/ejar.1997.406296
SHAARAWY, M., Jen, H., SHWANG, X., SETHER, D. (1997). 'REACTIVITY OF SOME HAWAIIAN BANANA BUNCHY TOP DISEASED SAMPLES USING DAS-ELISA AND A DIGOXIGENIN LABELLED DNA PROBE', Egyptian Journal of Agricultural Research, 75(3), pp. 515-527. doi: 10.21608/ejar.1997.406296
SHAARAWY, M., Jen, H., SHWANG, X., SETHER, D. REACTIVITY OF SOME HAWAIIAN BANANA BUNCHY TOP DISEASED SAMPLES USING DAS-ELISA AND A DIGOXIGENIN LABELLED DNA PROBE. Egyptian Journal of Agricultural Research, 1997; 75(3): 515-527. doi: 10.21608/ejar.1997.406296
REACTIVITY OF SOME HAWAIIAN BANANA BUNCHY TOP DISEASED SAMPLES USING DAS-ELISA AND A DIGOXIGENIN LABELLED DNA PROBE
1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
2University of Hawaii, Manoa, Plant Viral Lab., USA.
Abstract
Some Hawaiian bananas infected with BBTD gave a positive reaction using DAS-ELISA and Dot-Blot, a digoxigenin-labeled DNA probe. However, DAS-ELISA was more sensitive with diluted crude sap than the digoxigenin-labeled DNA probe (Dot-Blot), but both assays were similar at a 1/5 dilution of infected banana sap in all cases. Therefore, a digoxigenin-labeled DNA probe could be used alongside DAS-ELISA for routine diagnosis of BBTV in suspicious banana tissue for phytosanitary purposes.
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