DETERMINATION OF ANTIGENIC VARIATION AMONG STRAINS OF FLEXIBACTER COLUMNARIS ISOLATED FROM FRESHWATER FISH

Document Type : Original Article

Authors

Animal Health Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt.

Abstract

The present study was carried out on forty fish of Oreochromis niloticus and Clarias lazera (twenty for each) collected from River Nile, at Moneeb, Giza Governorate. Three isolates of Flexibacter columnaris were isolated from Oreochromis niloticus as well as 5 isolates from Clarias lazera. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SOS-PAGE) was done on the recovered isolates from both hosts for determination of their antigenic variation. Flexibacter columnaris isolated from both Oreochromis niloticus and from Clarias lazera showed three clear bands between 39.59-50.13 KO, and that isolated from Clarias lazera showed a characteristic band at 29.24 KO.