CHARACTERIZATION OF TRI-CHOMONAS FOETUS ANTIGEN FOR DIAGNOSTIC PURPOSES

Document Type : Original Article

Authors

1 Animal Reproduction Research Institute, Agricultural Research Centre, Giza, Egypt

2 Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Abstract

A comparison between serological and culture methods for diagnostis of Tr.foetus in cattle has been worked out . From 159 cows, only 11 serum samples were found to harbour anti-Tr.foetus antibodies by ELISA (6.9%). Samples of vaginal mucus from 12 animals were proved to be positive (7.5%) by culture method. The Three organisms were recovered from 25 mucus samples (15.7%). SDS-PAGE of Tr.foetus revealed the occurrence of 21 protein fractions with molecular weight varying from <17 KD to >130 KD.