EFFECT OF SODIUM CHLORIDE ON THE DIFFERENT MORPHOGENETIC PARAMETERS OF PAULOWNIA TOMENTOSA VAR. PALLIDA CULTURED IN VITRO

Document Type : Original Article

Authors

1 Horticultural Department, Faculty of Agriculture, Floriculture, Cairo University, Giza, Egypt.

2 Tissue Culture Laboratory, Agriculture Development Systems Project (ADSP), Agricultural Research Centre, Giza, Egypt.

Abstract

Nodal cutting explants of Paulownia tomentosa var. pallida were cultured in shooting media (MS + 5.0 mg/1 Zip) supplemented with NaCI at 0, 05, 150, 4550, 3600, 7200 and 14400 mg/l. The survival plant-lets were divided into nodal cutting (2cm) and cultured in rooting media (1/2 MS + 1.0 mg/1 IBA) supplemented with NaCI at 0, 50, 150, 4550 and 1800 mg/1. Shoot formation of Paulownia in shooting media in­creased with increasing NaCl concentration up to 1800 mg/1. where as high NaCI at 3600, 7200 and 14400 mg/1 decreased the growth and development. Root formations in rooting media decreased by NaCI at all concentrations compared to the control medium, whereas callus forma­tion enhanced by lower NaCI at 50 and 150 mg/1 (6 and 4 times re­spectively compared to control medium). At high concentrations of NaCI, the plantlets started to wilt.

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