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MOSSALAM, I., EL-REFAII, A., MOUSA, W., WAHBA, A. (1995). STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA. Egyptian Journal of Agricultural Research, 73(4), 1123-1131. doi: 10.21608/ejar.1995.442918
ISMAIL MOSSALAM; ABDEL-HAY H. EL-REFAII; WAHED M. MOUSA; AHMED A. WAHBA. "STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA". Egyptian Journal of Agricultural Research, 73, 4, 1995, 1123-1131. doi: 10.21608/ejar.1995.442918
MOSSALAM, I., EL-REFAII, A., MOUSA, W., WAHBA, A. (1995). 'STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA', Egyptian Journal of Agricultural Research, 73(4), pp. 1123-1131. doi: 10.21608/ejar.1995.442918
MOSSALAM, I., EL-REFAII, A., MOUSA, W., WAHBA, A. STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA. Egyptian Journal of Agricultural Research, 1995; 73(4): 1123-1131. doi: 10.21608/ejar.1995.442918

STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA

Article 47, Volume 73, Issue 4, December 1995, Page 1123-1131  XML PDF (2.24 MB)
Document Type: Original Article
DOI: 10.21608/ejar.1995.442918
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Authors
ISMAIL MOSSALAM1; ABDEL-HAY H. EL-REFAII2; WAHED M. MOUSA3; AHMED A. WAHBA2
1Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
2Animal Health Research Institute, Agricultural Research Centre, Giza, Egypt.
3Faculty of Veterinary Medicine, Zagazig University, Egypt
Abstract
The value of Enzyme Linked Immunosorbent Assay (ELISA) for di­agnosing natural buffaloes and camels sarcocystosis, as well as experi­mental sheep sarcocystosis were studied. In ELISA, the sensitivity and specificity were 80.85% and 53.19% for diagnosing buffaloes naturally infected with S.fusiformis, while the sensitivity and specificity were 93.62% and 95.74%, respectively for diagnosing Sarcocystis in naturally infected camels. The characterization of bradyzoites antigens (buffaloes and cam­els origin) using SOS-PAGE were studied. Concerning the experimentally infected sheep with sarcocystosis (buffaloes and camels origin), the an­tibody levels appeared at 2 weeks post-infection and gradually increased till 6 weeks post-infection and nearly at constant level till the end of ex­periment (8 weeks post-infection).
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