STUDIES ON SARCOCYTOSIS AMONG SOME FARM ANIMALS II- EVALUATION OF BRADYZOITES ANTIGENS BY ELISA

Document Type : Original Article

Authors

1 Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

2 Animal Health Research Institute, Agricultural Research Centre, Giza, Egypt.

3 Faculty of Veterinary Medicine, Zagazig University, Egypt

Abstract

The value of Enzyme Linked Immunosorbent Assay (ELISA) for di­agnosing natural buffaloes and camels sarcocystosis, as well as experi­mental sheep sarcocystosis were studied. In ELISA, the sensitivity and specificity were 80.85% and 53.19% for diagnosing buffaloes naturally infected with S.fusiformis, while the sensitivity and specificity were 93.62% and 95.74%, respectively for diagnosing Sarcocystis in naturally infected camels. The characterization of bradyzoites antigens (buffaloes and cam­els origin) using SOS-PAGE were studied. Concerning the experimentally infected sheep with sarcocystosis (buffaloes and camels origin), the an­tibody levels appeared at 2 weeks post-infection and gradually increased till 6 weeks post-infection and nearly at constant level till the end of ex­periment (8 weeks post-infection).

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