NEONATAL CALF PROBLEMS DUE TO SOME VIRAL INFECTIONS

Document Type : Original Article

Authors

1 Faculty of veterinary Medicine, Cairo University, Giza, Egypt

2 Animal Health Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt

3 Serum and Vaccine production Research Institute, Cairo, Egypt

Abstract

Seroprevalence study has been done using Enzyme Immuno As‌say (ELISA) for the detection of specific antibodies to some viruses known to be recorded in Egypt. A total of 451 serum samples were col‌lected from calves aging 2 weeks to 8 months from different governo‌rates of Egypt and were tested for antibodies to RP (56 %), FMD (36 %) , BVD (35 %), IBR (33 % ) and RVF (0%) The Prevalence rates werre 56 % , 36 % , 35% , 33 % and 0 % to the previously mentioned viruses respectively, suggesting a passive transfer of colostrum antibodies to the calves, or active exposure to the viruses specially those where vaccines are not available (BVD and IBR vi‌ruses). Trials have been done for isolation and identification of viral agent from nasal and rectal swabs collected from diseased or contact calves in tested governorates of Egypt. Out of 121 swabs collected, only 9 viral agents have been isolat‌ed and identified serologically by IFA and EIA techniques using reference sera available. Four out of nine isolates were + ve to enteroviruses, three were positive to IBR virus and one was P1-3 positive, while the remaining one was negative to all reference sera used. The pathogenicity of these isolates was confirmed by experimen‌tal inoculation of susceptible animals. All isolates were recovered from nasal and /or rectal swabs 5 days after experimental infection in susceptible calves.