CALLUS INDUCTION AND ORGAN DIFFERENTIATION IN THE TISSUE CULTURE OF FICUS SPP.

ARAFA, AZZA S.; MOHAMED, BARLANT R.; IBRAHIM, IBRAHIM A.; NASREL-DIN, TAUMOR M.

doi:10.21608/ejar.1993.467482

CALLUS INDUCTION AND ORGAN DIFFERENTIATION IN THE TISSUE CULTURE OF FICUS SPP.

Document Type : Original Article

Authors

¹ Department of Horticulture, Faculty of Agriculture, Cairo University, Giza, Egypt.

² Horticultural Research Institute, Agricultural Research Centre, Cairo, Egypt

³ Plant Diseases Research Institute, Agricultural Research Centre, Giza, Egypt.

10.21608/ejar.1993.467482

Abstract

Leaf fragments of Ficus spp. were excised, surface sterilized, and inoculated on Murashige and Shoog medium (MS) supplemented with 170 mg/1 NaH₂Po₄, 80 mg/I addenine sulfate, 30 mg/I isopentyl adenine (tip) and 0.3 mg/I indole acetic acid (IAA). The highest survival percentage was resulted from Ficus benjamina var Comosa (91.7%) and the lowest one was found with Ficus benjamina var Goldpricn (85%) Callus formation was 100% for all survived leaf section of Ficus benjamina var Califfornia, Exotica, Goldprincis and the lowest was found with Ficus benjamina var Comosa (36.5%). On the other hand, Ficus niticla had no ability to form callus on the medium under experimental conditions, Gradual, increase in callus weight were occurred in case of Ficus benjamina var Starlight. On the other hand, Ficus benjamina Callus failed to induce organogensis in different media used. Whereas Ficus lyrata callus differentiated to plantlets in MS medium containing high concentration of 2 ip (30 mg/I).