Home Articles List Article Information
Egyptian Journal of Agricultural Research
Journal Archive
Volume Volume 103 (2025)
Volume Volume 102 (2024)
Volume Volume 101 (2023)
Volume Volume 100 (2022)
Volume Volume 99 (2021)
Volume Volume 98 (2020)
Volume Volume 97 (2019)
Volume Volume 96 (2018)
Volume Volume 95 (2017)
Volume Volume 94 (2016)
Volume Volume 93 (2015)
Volume Volume 92 (2014)
Volume Volume 91 (2013)
Volume Volume 90 (2012)
Volume Volume 89 (2011)
Volume Volume 88 (2010)
Volume Volume 87 (2009)
Volume Volume 86 (2008)
Volume Volume 85 (2007)
Volume Volume 84 (2006)
Volume Volume 83 (2005)
Volume Volume 82 (2004)
Volume Volume 81 (2003)
Volume Volume 80 (2002)
Volume Volume 79 (2001)
Volume Volume 78 (2000)
Volume Volume 77 (1999)
Volume Volume 76 (1998)
Volume Volume 75 (1997)
YOUSSEF, E., TAWFIK, H. (2011). IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50. Egyptian Journal of Agricultural Research, 89(2), 697-708. doi: 10.21608/ejar.2011.176060
ELHAM A. YOUSSEF; HODA E. TAWFIK. "IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50". Egyptian Journal of Agricultural Research, 89, 2, 2011, 697-708. doi: 10.21608/ejar.2011.176060
YOUSSEF, E., TAWFIK, H. (2011). 'IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50', Egyptian Journal of Agricultural Research, 89(2), pp. 697-708. doi: 10.21608/ejar.2011.176060
YOUSSEF, E., TAWFIK, H. IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50. Egyptian Journal of Agricultural Research, 2011; 89(2): 697-708. doi: 10.21608/ejar.2011.176060

IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50

Article 22, Volume 89, Issue 2, July 2011, Page 697-708  XML PDF (253.35 K)
Document Type: Original Article
DOI: 10.21608/ejar.2011.176060
View on SCiNiTO View on SCiNiTO
Authors
ELHAM A. YOUSSEF; HODA E. TAWFIK
Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt.
Abstract
Montanide ISA50 was used as an adjuvant for preparation of inactivated rabbit pasteurellosis vaccine, and compared with the commercially produced oil inactivated rabbit pasteurellosis vaccine adjuvanted with mineral oil. Two groups of rabbits were inoculated separately by first dose of these vaccines followed by second dose after 21 days from the first dose, the mineral oil used for first group and ISA50 used for the second group as adjuvant, meanwhile, the third group was left as non-vaccinated control group. Cellular immunity was evaluated by using cell proliferation test and macrophage activity test (phagocytic percentage, phagocytic index). Humoral immune response was evaluated by using indirect haemagglutination test (IHA) and enzyme linked immunosorbent assay (ELISA). The results of the aforementioned tests proved that the two types of adjuvant induced protective titre against the diseases, but Montanide ISA50 oil adjuvant gave higher and longer duration of antibody level than the mineral oil. Challenge test and protection test in rabbit and mice gave similar results.
Statistics
Article View: 90
PDF Download: 343