El-sayed, M., Assi, H., Mekky, S., Soliman, H. (2022). Molecular detection of some growth-hormone genes in chickens. Egyptian Journal of Agricultural Research, 100(2), 193-203. doi: 10.21608/ejar.2022.101849.1163
Mohammed A. El-sayed; Heba A.E.M. Assi; Saadeya S. Mekky; Hesham A.M. Soliman. "Molecular detection of some growth-hormone genes in chickens". Egyptian Journal of Agricultural Research, 100, 2, 2022, 193-203. doi: 10.21608/ejar.2022.101849.1163
El-sayed, M., Assi, H., Mekky, S., Soliman, H. (2022). 'Molecular detection of some growth-hormone genes in chickens', Egyptian Journal of Agricultural Research, 100(2), pp. 193-203. doi: 10.21608/ejar.2022.101849.1163
El-sayed, M., Assi, H., Mekky, S., Soliman, H. Molecular detection of some growth-hormone genes in chickens. Egyptian Journal of Agricultural Research, 2022; 100(2): 193-203. doi: 10.21608/ejar.2022.101849.1163
Molecular detection of some growth-hormone genes in chickens
1Animal Genetic Resources Department, National Gene Bank, Agricultural Research Center, Giza, Egypt
2Animal Production Research Institute, Agricultural Research Center, Giza, Egypt
3Animal and Poultry Breeding Department, Desert Research Center, Matariya, Cairo, Egypt
4Animal and Poultry Breeding Department, Desert Research Center, Cairo, Egypt
Abstract
Due to the fact that the chicken growth hormone gene (cGH) is critical for controlling development and metabolism, there could be a link between GH polymorphisms and economic features in chickens. The objective of this study was to detect the single nucleotide polymorphisms (SNPs) in the GH and insulin-like growth factor-binding protein 2 (IGFBP2) genes, and to investigate their associations with body weight (BW). Three hundred individuals (100 birds for each commercial strains Sasso and Ross and one local breed; Baladi) were assayed. And the three primers were amplification with a total of 54 samples from three genotypes (Ross=18, Sasso=18 and Baladi=18). A total of SNPs sequenced was 138,106bp consisting of 13,265bp coding regions and 124,841bp non-coding regions. A total of 197 SNPs were identified, with 34 in coding SNPs regions and 163 in non-coding SNPs regions. The values of variation in coding regions, transitions accounted for 53% (18),26% (9) and 21% (7),while in non-coding regions were 27% (111),33% (132) and 40% (163) in Ross, Sasso and Baladi for three genes. This result is most likely due to the high, medium, and low body weights of Ross, Sasso, and Baladi breed. The higher frequency of A↔G and A↔C and lower frequency of G↔T may be related to higher body weight in the Ross strain. While, the higher frequency of A↔C, C↔T and lower G↔T may be related to lower body weight with Baladi breed. Our study confirms the analyzed three genes affecting meat production in poultry.
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