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YOUSEF, N., DABA, A., EL-KATTAN, M. (2005). MYCELIAL AND EXO-POLYSACCHARIDES PRODUCTION BY SUBMERGED CULTURE OF THE EDIBLE MUSHROOM PLEUROTUS SPECIES. Egyptian Journal of Agricultural Research, 83(2), 1065-1074. doi: 10.21608/ejar.2005.246517
NAHED S. YOUSEF; AUMAN S. DABA; MOUSTAFA H. EL-KATTAN. "MYCELIAL AND EXO-POLYSACCHARIDES PRODUCTION BY SUBMERGED CULTURE OF THE EDIBLE MUSHROOM PLEUROTUS SPECIES". Egyptian Journal of Agricultural Research, 83, 2, 2005, 1065-1074. doi: 10.21608/ejar.2005.246517
YOUSEF, N., DABA, A., EL-KATTAN, M. (2005). 'MYCELIAL AND EXO-POLYSACCHARIDES PRODUCTION BY SUBMERGED CULTURE OF THE EDIBLE MUSHROOM PLEUROTUS SPECIES', Egyptian Journal of Agricultural Research, 83(2), pp. 1065-1074. doi: 10.21608/ejar.2005.246517
YOUSEF, N., DABA, A., EL-KATTAN, M. MYCELIAL AND EXO-POLYSACCHARIDES PRODUCTION BY SUBMERGED CULTURE OF THE EDIBLE MUSHROOM PLEUROTUS SPECIES. Egyptian Journal of Agricultural Research, 2005; 83(2): 1065-1074. doi: 10.21608/ejar.2005.246517

MYCELIAL AND EXO-POLYSACCHARIDES PRODUCTION BY SUBMERGED CULTURE OF THE EDIBLE MUSHROOM PLEUROTUS SPECIES

Article 35, Volume 83, Issue 2, June 2005, Page 1065-1074  XML PDF (1.96 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2005.246517
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Authors
NAHED S. YOUSEF1; AUMAN S. DABA2; MOUSTAFA H. EL-KATTAN3
1Faculty of Home Economic, Al-Azhar University, Tanta, Egypt
2Mubarak City for Scientific Research, Genetic Engineering and Biotechnology Research Institute, Alexandria, Egypt
3Central Laboratory of Agricultural climate, Agricultural Research Center, Dokki, Giza, Egypt
Abstract
Submerged cultures of four Pleurotus species, a popular mushroom cultivated in Egypt, was used for production of bio­active protein-bound polysaccharides. The maximum concentration of mycelia! biomass approximated 10 g1' with 2.76 g/1'1 protein in Pleurotus &tea after 14 days incubation in shake flask culture. The exopolysaccharide (EPS) production after the same incubation period was higher in P. sajor-caju and P. Aorida (1.4 g/r1) than P. ostreatus and P. erengii (0.8 g/1-1). The main components of the isolated polysaccharides were glucans as shown by the NMR spectra. The electrophoretic pattern indicates that the separated polysaccharides are bound to proteins and the molecular weight ranges from 10 to 200 KD. The polysaccharide showed a notable proliferation activity for peripheral blood mononudear cells (PBMC).
Keywords
mushroom; polysaccharide; Pleurotus; submerged culture; mycelial biomass
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