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EL-REFAII, M., WAHBA, A., SHEHAB, G. (2002). PARASITOLOGICAL AND PATHOLOGICAL STUDIES ON NEOSPORA CAN1NUM IN EXPERIMENTALLY INFECTED DOGS. Egyptian Journal of Agricultural Research, 80(3), 1355-1372. doi: 10.21608/ejar.2002.312661
MAGDA A. H. EL-REFAII; AHMED A. WAHBA; GEHAN J. SHEHAB. "PARASITOLOGICAL AND PATHOLOGICAL STUDIES ON NEOSPORA CAN1NUM IN EXPERIMENTALLY INFECTED DOGS". Egyptian Journal of Agricultural Research, 80, 3, 2002, 1355-1372. doi: 10.21608/ejar.2002.312661
EL-REFAII, M., WAHBA, A., SHEHAB, G. (2002). 'PARASITOLOGICAL AND PATHOLOGICAL STUDIES ON NEOSPORA CAN1NUM IN EXPERIMENTALLY INFECTED DOGS', Egyptian Journal of Agricultural Research, 80(3), pp. 1355-1372. doi: 10.21608/ejar.2002.312661
EL-REFAII, M., WAHBA, A., SHEHAB, G. PARASITOLOGICAL AND PATHOLOGICAL STUDIES ON NEOSPORA CAN1NUM IN EXPERIMENTALLY INFECTED DOGS. Egyptian Journal of Agricultural Research, 2002; 80(3): 1355-1372. doi: 10.21608/ejar.2002.312661

PARASITOLOGICAL AND PATHOLOGICAL STUDIES ON NEOSPORA CAN1NUM IN EXPERIMENTALLY INFECTED DOGS

Article 29, Volume 80, Issue 3, September 2002, Page 1355-1372  XML PDF (5.82 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2002.312661
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Authors
MAGDA A. H. EL-REFAII; AHMED A. WAHBA; GEHAN J. SHEHAB
Animal Health Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
Neospora caninum is a protozoan parasite. It was isolated from brain of naturally infected rats and fed on puppies. Five to ten days post-infection, puppies began to excrete unsporulated oocysts. Sporulat­ed oocycts were fed to rats. After death, fresh smears from intestines of puppies and brain smears stained with Giemsa stain from rats were taken for parasitological examination. Meanwhile, tissue specimens from the internal organs wer taken from puppies and rats for histopathological examination. However, only brains from rats were taken for electron mi­croscopy examination. Histopathological examination revealed meningoencephalomyeli­tis, hepatitis, nephrosis and occasional interstitial pneumonia in puppies and rats, in addition to myocarditis and myocitis in puppies. Tissue cysts and tachyzoites were present in lesions in the brain of rats. In puppies, tachyzoites were seen in the brain and in parasitophorous vacuoles with­in the cytoplasm of epithelium lining the small intestine. Electron microscopy examination of the brain of rats revealed ta­chyzoites and tissue cysts. Parasitological and pathological importance of N. caninum in pup­pies and rats was fully discussed.
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